- 発 表 論 文 等 -

●2018年●

Endo S, Maeda T, Kawame T, Iwai N, Wachi M. (2018) RNase E/G-dependent degradation of metE mRNA, encoding methionine synthase, in Corynebacterium glutamicum. J. Gen. Appl. Microbiol., 65(1), 47-51.

Theresia N M, Aida K, Takada A, Iwai N, Wachi M. (2018) Effects of EGTA on cell surface structures of Corynebacterium glutamicum. Arch. Microbiol., 200(2), 281-287.

●2017年●

Maeda T, Tanaka Y, Wachi M, Inui M. (2017) Polynucleotide phosphorylase, RNase E/G, and YbeY are involved in the maturation of 4.5S RNA in Corynebacterium glutamicum. J. Bacteriol., 199, e00798-16.

Hirasawa T, Wachi M. (2017) Glutamate fermentation-2: Mechanism of L-glutamate overproduction in Corynebacterium glutamicum. Adv. Biochem. Eng. Biotechnol., 159, 57-72. (Review) 

●2015年●

Fang MY, Zhang C, Yang S, Cui JY, Jiang PX, Lou K, Wachi M, Xing XH. (2015) High crude violacein production from glucose by Escherichia coli engineered with interactive control of tryptophan pathway and violacein biosynthetic pathway. Microb. Cell Fact., 14, 8.

Yano K, Wachi M, Tsuchida S, Kitazume T, Iwai N. (2015) Degradation of benzotrifluoride via the dioxygenase pathway in Rhodococcus sp. 065240. Biosci. Biotechnol. Biochem., 79, 496-504.

●2014年●

Uchida M, Hirasawa T, Wachi M.(2014) Characterization of a Corynebacterium glutamicum dnaB mutant that shows temperature-sensitive growth and mini-cell formation. Arch. Microbiol., 196, 871-879.

Kitahara Y, Yin T, Zhao X, Wachi M, Du W, Liu D. (2014) Isolation of oleaginous yeast (Rhodosporidium toruloides) mutants tolerant of sugarcane bagasse hydrolysate. Biosci. Biotechnol. Biochem., 78, 336-342.

Matsuda Y, Itaya H, Kitahara Y, Theresia NM, Kutukova EA, Yomantas YA, Date M, Kikuchi Y, Wachi M. (2014) Double mutation of cell wall proteins CspB and PBP1a increases secretion of the antibody Fab fragment from Corynebacterium glutamicum. Microb. Cell Fact., 13, 56.

●2013年●

Ito K, Hamasaki K, Kayamori A, Nguyen PAT, Amagai K, Wachi M. (2013) A secondary structure in the 5’ untranslated region of adhE mRNA required for RNase G-dependent regulation. Biosci. Biotechnol. Biochem., 77, 2473-2479.

Supkulsutra T, Maeda T, Kumagai K, Wachi M. (2013) A role of the transcriptional regulator LldR (NCgl2814) in glutamate metabolism under biotin-limited conditions in Corynebacterium glutamicum. J. Gen. Appl. Microbiol., 59, 207-214.

Yamashita C, Hashimoto K, Kumagai K, Maeda T, Takada A, Yabe I, Kawasaki H, Wachi M. (2013) L-Glutamate secretion by the N-terminal domain of the Corynebacterium glutamicum NCgl1221 mechanosensitive channel. Biosci. Biotechnol. Biochem., 77, 1008-1013.

Li J, Sakai N, Watanabe M, Hotta E, Wachi M. (2013) Study on plasma agent effect of a direct-current atmospheric pressure oxygen-plasma jet on inactivation of E. coli using mutants. IEEE Transactions on Plasma Science. 41, 935-941.

●2012年●

Maeda T, Wachi M. (2012) 3' Untranslated region-dependent degradation of the aceA mRNA, encoding the glyoxylate cycle enzyme isocitrate lyase, by RNase E/G in Corynebacterium glutamicum. Appl. Environ. Microbiol., 78, 8753-8761.

Maeda T, Wachi M. (2012) Corynebacterium glutamicum RNase E/G-type endoribonuclease encoded by NCgl2281 is involved in the 5' maturation of 5S rRNA. Arch. Microbiol., 194, 65-73.

川崎寿、和地正明 (2012) Corynebacterium glutamicumのグルタミン酸排出チャネルの発見とその有用物質生産への展開、化学と生物、50, 441-449.

●2011年●

Wachi M. (2011) Amino acid exporters in Corynebactrium glutamicum. Microbiol. Monogr., 23, 335-349.

Deininger KN, Horikawa A, Kitko RD, Tatsumi R, Rosner JL, Wachi M, Slonczewski JL. (2011) A requirement of TolC and MDR efflux pumps for acid adaptation and GadAB induction in Escherichia coli. PLoS One. 6:e18960.

●2010年●

Takeshita, R., Ito, H., and Wachi, M. (2010) A role of the cspA gene encoding a mycolyltransferase in the growth under alkaline conditions of Corynebacterium glutamicum. Biosci. Biotechnol. Biochem., 74(8), 1617-1623.

和地正明 (2010) 大腸菌RNase Gによる解糖系の制御. バイオサイエンスとインダストリー, 68(1), 34-38.

Heinemann, I. U., Schulz, C., Schubert, W-D., Heinz, D. W., Wang, Y-G., Kobayashi, Y., Awa, Y., Wachi, M., Jahn, D., and Jahn, M. (2010) Structure of the heme biosynthetic Pseudomonas Aeruginosa porphobilinogen synthase in complex with the antibiotic alaremycin. Antimicrob. Agents Chemother., 54(1), 267-272

Kim, J., Fukuda, H., Hirasawa, T., Nagahisa, K., Nagai, K., Wachi, M., and Shimizu, H. (2010) Requirement of de novo synthesis of the OdhI protein in penicillin-induced glutamate production by Corynebacterium glutamicum. Appl. Microbiol. Biotechnol., 86, 911-920.

●2009年●

Maeda, T., Sakai, T., and Wachi, M. (2009) The Corynebacterium glutamicum NCgl2281 gene encoding an RNase E/G family endoribonuclease can complement the Escherichia coli rng::cat mutation but not the rne-1 mutation. Biosci. Biotechnol. Biochem., 73(10), 2281-2286.

●2008年●

中村純、和地正明 (2008) Corynebacterium glutamicumによるグルタミン酸発酵生産のメカニズム, FFIジャーナル, 213(12), 1103-1109.

和地正明、岩井伯隆 (2008) 細菌の細胞骨格を標的とうる抗菌剤の開発. 蛋白質 核酸 酵素, 53(13), 1765-1770.

Tatsumi, R., and Wachi, M. (2008) TolC-dependent exclusion of porphyrins in Escherichia coli. J. Bacteriol., 190(18), 6228-6233.

Noguchi, N., Yanagimoto, K., Nakaminami, H., Wakabayashi, M., Iwai, N., Wachi, M., and Sasatsu, M. (2008) Anti-infectious effect of S-benzylisothiourea compound A22, which inhibits the actin-like protein, MreB, in Shigella flexneri. Biol. Pharm. Bull., 31(7), 1327-1332.

Sato, H., Orishimo, K., Shirai, T., Hirasawa, T., Nagahisa, K., Shimizu, H., and Wachi, M. (2008) Distinct roles of two anaplerotic pathways in glutamate production induced by biotin limitation in Corynebacterium glutamicum. J. Biosci. Bioeng., 106(1), 51-58.

Gao YG, Suzuki H, Itou H, Zhou Y, Tanaka Y, Wachi M, Watanabe N, Tanaka I, Yao M. (2008) Structural and functional characterization of the LldR from Corynebacterium glutamicum: a transcriptional repressor involved in L-lactate and sugar utilization. Nucleic Acids Res., 36, 7110-7123.

●2007年●

Nakamura, J., Hirano, S., Ito, H., and Wachi, M. (2007) Mutations of the Corynebacterium glutamicum NCgl1221 gene, encoding a mechanosensitive channel homolog, induce L-glutamic acid production. Appl. Environ. Microbiol., 73(14), 4491-4498.

Sakai, T., Nakamura, N., Umitsuki, G., Nagai, K., and Wachi, M. (2007) Increased production of pyruvic acid by Escherichia coli RNase G mutants in combination with cra mutations. Appl. Microbiol. Biotechnol., 76, 183-192.

Takada, A., Umitsuki, G., Nagai, K., and Wachi, M. (2007) RNase E is required for induction of the glutamate-dependent acid resistance system in Escherichia coli. Biosci. Biotechnol. Biochem., 71(1), 158-164.

Nakagawa, H., Hasumi, K., Takami, M., Aida-Hyugaji, S., Woo, J.-T., Nagai, K., Ishikawa, T., and Wachi, M. (2007) Identification of two biologically crucial hydroxyl groups of (-)-epigallocatechin gallate in osteoclast culture. Biochem. Pharmacol., 73, 34-43.

和地正明 (2007) 阻害剤を使った解析から見えてきた細菌の有糸分裂装置?−アクチンホモログMreBが娘細胞への染色体分配に関与−. 化学と生物, 45(12), 812-813.

和地正明、岩井伯隆 (2007) 抗菌薬の新規標的としての細菌のアクチン様細胞骨格タンパク質. 日本細菌学会雑誌, 62(4), 397-404.

和地正明 (分担) (2007) タンパク質にタグを付ける, 今日からもの知りシリーズトコトンやさしいタンパク質の本(東京工業大学大学院生命理工学研究科編), 日刊工業新聞社, 100-101.

Horie, Y., Ito, Y., Ono, M., Moriwaki, N., Kato, H., Hamakubo, Y., Amano, T., Wachi, M., Shirai, M., and Asayama, M. (2007) Dark-induced mRNA instability involves RNase E/G-type endoribonuclease cleavage at the AU-box and SD sequences in cyanobacteria. Mol. Genet. Genomics., 278, 331-346.

Watanabe, T., Furukawa, S., Kawarai, T., Wachi, M., Ogihara, H., and Yamasaki, M. (2007) Cytoplasmic acidification may occur in high pressure carbon dioxide-treated Escherichia coli K12. Biosci. Biotechnol. Biochem., 71(10), 2522-2526.

Oyamada, Y., Yamagishi, J., Kihara, T., Yoshida, H., Wachi, M., and Ito, H. (2007) Mechanism of Inhibition of DNA Gyrase by ES-1273, a Novel DNA Gyrase Inhibitor. Microbiol. Immunol., 51(10), 977-84.

●2006年●

Wachi, M., Osaka, K., Kohama, T., Sasaki, K., Ohtsu, I., Iwai, N., Takada, A., and Nagai, K. (2006) Transcriptional analysis of the Escherichia coli mreBCD genes responsible for morphogenesis and chromosome segregation. Biosci. Biotechnol. Biochem., 70(11), 2712-2719.

Ito, H., Ura, A., Oyamada, Y., Tanitame, A., Yoshida, H., Yamada, S., Wachi, M., and Yamagishi, J. (2006) A 4-aminofurazan derivative-A189-inhibits assembly of bacterial cell division protein FtsZ in vitro and in vivo. Microbiol. Immunol., 50(10), 759-764.

Nakamura, J., Kanno, S., Kimura, E., Matsui, K., Nakamatsu, T., and Wachi, M. (2006) Temperature-sensitive cloning vector for Corynebacterium glutamicum.Plasmid, 56(3), 179-186.

Oyamada, Y., Ito, H., Fujimoto-Nakamura, M., Tanitame, A., Iwai;, N., Nagai, K., Yamagishi, J., and Wachi, M. (2006) Anucleate cell blue assay : a useful tool for identifying novel type II topoisomerase inhibitors. Antimicrob. Agents Chemother., 50(1), 348-350.

Kruse, T., Blagoev, B., Lobner-Olesen, A., Wachi, M., Sasaki, K. Iwai, N., Mann, M., and Gerdes, K. (2006) Actin homolog MreB and RNA polymerase interact and are both required for chromosome segregation in Escherichia coli. Genes & Dev., 20, 113-124.

Wang, Y.-G., Wachi, M., and Kobayashi, Y. (2006) Synthesis of Alaremycin. Synlett, 3(46), 481-483.

●2005年●

Awa, Y., Iwai, N., Ueda, T., Suzuki, K., Asano, S., Yamagishi, J., Nagai, K., and Wachi, M. (2005) Isolation of a new antibiotic, alaremaycin, structurally related to 5-aminolevulinic acid from Streptomyces sp. A012304. Biosci. Biotechnol. Biochem., 69(9), 1721-1725.

Kumagai, Y., Hirasawa, T., Hayakawa, K., Nagai, K., and Wachi, M. (2005) Fluorescent phospholipid analogs as microscopic probes for detection of the mycolic acid-containing layer in Corynebacterium glutamicum : Detecting alterations in the mycolic acid-containing layer following ethambutol treatment. Biosci. Biotechnol. Biochem., 69(11), 2051-2056.

Itou, H., Okada, U., Suzuki, H., Yao, M., Wachi, M., Watanabe, N., Tanaka, I. (2005) The CGL2612 protein from Corynebacterium glutamicum is a drug resistance-related transcriptional repressor: structural and functional analysis of a newly identified transcription factor from genomic DNA analysis. J. Biol. Chem., 280(46), 38711-38719.

Takada, A., Nagai, K., and Wachi, M. (2005) A decreased level of FtsZ is responsiblefor inviability of RNase E-deficient cells. Genes Cells, 10, 733-741.

Gitai, Z., Dye, N.A., Reisenauer, A., Wachi, M., and Shapiro, L. (2005) MreB actin-mediated segregation of a specific region of a bacterial chromosome. Cell, 120, 329-341. 

●2004年●

Iwai, N., Ebata, T., Nagura, H., Kitazume, T., Nagai, K., and Wachi, M. (2004) Structure-activity relationship of S-benzylisothiourea derivatives to induce spherical cells in Escherichia coli. Biosci. Biotechnol. Biochem., 68(11), 2265-2269.

Tanitame, A., Oyamada, Y., Ofuji, K., Fujimoto, M., Suzuki, K., Ueda,T., Terauchi, H., Kawasaki, M., Nagai, K., Wachi, M., and Yamagishi, J.(2004) Synthesis and antibacterial activity of novel and potent DNA gyrase inhibitors with azole ring. Bioorg Med Chem Lett., 12, 5515-5524.

Ohtsu, I., Kakuda, N., Tsukagoshi, N., Dokyu, N., Takagi, H., Wachi,M., and Aono, R. (2004) Transcriptional analysis of the ostA / imp geneinvolved in organic solvent sensitivity in Escherichia coli. Biosci. Biotechnol. Biochem., 68(2), 458-461.

Ogino, H., Wachi, M., Ishii, A., Iwai, N., Nishida, T., Yamada, S.,Nagai, K., and Sugai, M. (2004) FtsZ-dependent localization of GroEL protein at possible division sites. Genes Cells, 9, 765-771.

和地正明 (2004) 大腸菌の細胞分裂とGroEL. 蛋白質 核酸 酵素, 49(7), 853.

Nakagawa, H., Hasumi, K., Woo, J-T., Nagai, K., and Wachi, M. (2004) Generation of hydrogen peroxide primarily contributes to the induction of Fe(III)-dependent apoptosis in Jurkat cells by (-)-epigallocatechin gallate. Carcinogenesis,25(9), 1567-1574.

Ishii, A., Sato, T., Wachi, M., Nagai, K., and C. Kato, D. (2004) Effectsof high hydrostatic pressure on bacterial cytoskeleton FtsZ polymers invivo and in vitro. Microbiology, 150, 1965-1972.

Tanitame, A., Oyamada, Y., Ofuji, K., Kyoya, Y., Suzuki, K., Ito, H., Kawasaki, M., Nagai, K., Wachi, M., Yamagishi, J. (2004a) Design, synthesis and antibacterial activity of a novel series of potent DNA gyrase inhibitor. Pyrazole derivatives. J. Med. Chem., 47(14), 3693-3696.

Tanitame, A., Oyamada, Y., Ofuji, K., Suzuki, K., Ito, H., Kawasaki, M., Wachi, M., Yamagishi, J. (2004b) Potent DNA gyrase inhibitors; novel 5-vinylpyrazoleanalogues with Gram-positive antibacterial activity. Bioorg Med Chem Lett.,14(11), 2863-2866.

Tanitame, A., Oyamada, Y., Ofuji, K., Kyoya, Y., Suzuki, K., Ito, H., Kawasaki, M., Nagai, K., Wachi, M., Yamagishi, J. (2004c) Design, synthesis and structure-activity relationship studies of novel indazole analogues as DNA gyrase inhibitors with Gram-positive antibacterial activity. Bioorg. Med. Chem. Lett., 14(11), 2857-2862.

Kawarai, T., Wachi, M., Ogino, H., Furukawa, S., Suzuki, K., Ogihara, H., and Yamasaki, M. (2004) SulA-independent filamentation of Escherichia coli during growth after release from high hydrostatic pressure treatment. Appl. Microbiol. Biotechnol., 64(2), 255-262.

●2003年●

Nakagawa, H., Takami, M., Udagawa, N., Sawae, Y., Suda, K., Sasaki, T., Takahashi, N., Wachi, M., Nagai, K., Woo, J.-T. (2003) Destruxins, cyclodepsipeptides, block the formation of actin rings and prominent clear zones and ruffled borders in osteoclasts. Bone, 33(3), 443-455.

Inagawa, T., Okamoto, S., Wachi, M., Ochi, K. (2003) RNase ES of Streptomyces coelicolor A3(2) can complement the rne and rng mutations in Escherichia coli. Biosci. Biotechnol. Biochem., 67(8), 1767-1771.

Hirasawa, T., Kumagai, Y., Nagai, K., Wachi, M. (2003) A Corynebacterium glutamicum rnhA recG double mutant showing lysozyme-sensitivity, temperature-sensitivity growth, and UV-sensitivity ; Biosci. Biotechnol. Biochem., 67(11), 2416-2424.

●2002年●

Kaga, N., Umitsuki, G., Clark, D. P., Nagai, K., and Wachi, M. (2002) Extensive overproduction of the AdhE protein by rng mutations depends on mutations in the cra gene or in the Cra-box of the adhE promoter. Biochem. Biophys. Res. Commun., 295, 92-97.

Nakagawa, H., Wachi, M., Woo, J.-T., Kato, M., Kasai, S., Takahashi, F., Lee, I.-S., and Nagai, K. (2002) Fenton reaction is primarilyinvolved in a mechanism of (-)-epigallocatechin-3-gallate to induce osteoclastic cell death. Biochem. Biophys. Res. Commun., 292, 94-101.

Fujishima, H., Nishimura, A., Wachi, M., Takagi, H., Hirasawa, T., Teraoka, H., Nishimori, K., Kawabata, T., Nishikawa, K., and Nagai, K. (2002) kdsA mutations affect FtsZ-ring formation in Escherichia coli K-12. Microbiology,148, 103-112.

和地正明、永井和夫 (2002) 細菌の分裂機構とその進化. 遺伝, 別冊14号「細胞のミクロコスモス」, 6-12.

●2001年●

Wachi, M., Kaga, N., Umitsuki, G., Clark, D. P., and Nagai, K. (2001) A novel RNase G mutant that is defective in degradation of adhE mRNA but proficient in the processing of 16S rRNA precursor. Biochem. Biophys. Res. Commun., 289, 1301-1306.

Hirasawa, T., Wachi, M., and Nagai, K. (2001) L-Glutamate production by lysozyme-sensitive Corynebacterium glutamicum ltsA mutant strains. BMC Biotechnol., 1, 9.

Wachi, M., and Nagai, K. (2001) Escherichia coli ribonucleaseG. In A. W. Nicholson (ed.), Methods in Enzymology, "Ribonucleases", Academic Press, Inc., New York, 55-63.

Umitsuki, G., Wachi, M., Takada, A., Hikichi, T., and Nagai, K. (2001) Involvement of RNase G in in vivo mRNA metabolism in Escherichia coli. Genes Cells, 6, 403-410.

Wijayarathna, C. D., Wachi, M., and Nagai, K. (2001) Isolation of ftsI and murE genes involved in peptidoglycan synthesis from Corynebacterium glutamicum. Appl. Microbiol. Biotechnol.,55, 466-470.

和地正明 (2001) 大腸菌RNase G. 日本農芸化学会誌, 75, 121-127.

●2000年●

Hirasawa, T., Wachi, M., and Nagai, K. (2000) A mutation in the Corynebacterium glutamicum ltsA gene causes susceptibility to lysozyme, temperature-sensitive growth, and L-glutamate production J. Bacteriol., 182[10], 2696-2701.

●1999年●

Wachi, M., Wijayarathna, C. D., and Nagai, K. (1999) A murC gene from coryneform bacteria. Appl. Microbiol. Biotechnol., 51, 223-228.

Wachi, M., Umitsuki, G., Shimizu, M., Takada, A., and Nagai, K. (1999) Escherichia coli cafA gene encodes a novel RNase, designatedas RNase G, involved in processing of the 5' end of 16S rRNA. Biochem. Biophys. Res. Commun., 259, 483-488.

Wachi, M., Takada, A., and Nagai, K. (1999) Overproduction of the outer-membrane proteins FepA and FhuE responsible for iron transport in Escherichia coli hfq::cat mutant. Biochem. Biophys. Res. Commun., 264, 525-529.

Takada, A., Wachi, M., and Nagai, K. (1999) Negative regulatory role of the Escherichia coli hfq gene in cell division. Biochem. Biophys. Res. Commun., 266, 579-583.

Wachi, M., Iwai, N., Kunihisa, A., and Nagai, K. (1999) Irregular nuclear localization and anucleate cell production in Escherichia coli induced by a Ca2+ chelator, EGTA. Biochimie, 81, 909-913.

Nagano, K., Wachi, M., Takada, A., Takaku, F., Hirasawa, T., and Nagai, K. (1999) fcsA29 mutation is an allele of polA gene of Escherichia coli. Biosci. Biotechnol. Biochem., 63, 427-729.

Malathi, K. C., Wachi, M., and Nagai, K. (1999) Isolation of the murI gene from Brevibacterium lactofermentum ATCC13869encoding D-glutamate racemase. FEMS Microbiol. Lett., 175, 193-196.

●1998年●

Kijima, N., Goyal, D., Takada, A., Wachi, M., and Nagai, K. (1998) Induction of only limited elongation instead of filamentation by inhibition of cell division in Corynebacterium glutamicum. Appl.Microbiol. Biotechnol., 50, 227-232.

和地正明、永井和夫 (1998) コリネ型細菌の細胞複製機構の解析. バイオサイエンスとインダストリー, 56, 101-103.

和地正明、永井和夫 (1998) 大腸菌の細胞複製変異株. バイオサイエンスとインダストリー, 56, 227-228.

和地正明、永井和夫 (1998) 大腸菌の細胞周期と核様体タンパク質. バイオサイエンスとインダストリー, 56, 229-232.

永井和夫、和地正明、木嶋伸行、落合恵子 (1998) グルタミン酸生産菌Corynebacterium glutamicumの構造と細胞分裂. バイオサイエンスとインダストリー, 56, 83-84.

●1997年●

Wachi, M., Umitsuki, G. and Nagai, K. (1997) Functional relationship between Escherichia coli RNase E and the CafA protein. Mol. Gen. Genet., 253, 515-519.

Miki Kobayashi, Yoko Asai, Kazuhisa Hatakeyama, Kijima, N., Wachi, M., Nagai, K., and Yukawa, H. (1997) Cloning, sequencing and characterization of the ftsZ gene from coryneform bacteria. Biochem. Biophys. Res. Commun., 236, 383-388.

Takada, A., Wachi, M., Kaidow, A., Takamura, M., and Nagai, K. (1997) DNA binding properties of the hfq gene product of Escherichia coli. Biochem. Biophys. Res. Commun., 236, 576-579.

Nishimura, A., Moriya, S., Ukai, H., Nagai, K., Wachi, M., and Yamada, Y. (1997) Diadenosine 5',5'''-P1,P4-tetraphosphate (Ap4A) controls the timing of cell division in Escherichia coli. Genes Cells, 2, 401-413.

●1996年●

Goyal, D., Wachi, M., Kijima, N., Kobayashi, M., Yukawa, H., and Nagai, K. (1996) A cryptic plasmid pBL1 from Brevibacterium lactofermentum causes growth inhibition and filamentation in Escherichia coli. Plasmid, 36, 62-66.

 

- 特 許 -

 

和地正明、永井和夫 (2001) mRNAの安定制御によるタンパク質大量発現系. 国内特許,特願2001-42034.

和地正明 (2004) ピルビン酸の製造方法及びL-バリンの製造方法. 特願2004-154989, 特開2005-333855

 

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